Proteinase Inhibitors

Schirra, H.J., Renda, R.F., Anderson, M.A. and Craik, D.J. (2008) The structure of a disulphide mutant of the Nicotiana alata proteinase inhibitor T1-Stabilisation of the reactive site loop is critical for activity. (Accepted J. Mol. Biol. subject to revision).

Schirra, H.J., Anderson, M.A. and Craik, D.J.(2008) Structural refinement of insecticidal plant proteinase inhibitors from Nicotiana alata. Protein Pept. Lett. 15, 903-909.

Maheswaran, G., Pridmore, L., Franz, P. and Anderson, M.A. (2007) A proteinase inhibitor from Nicotiana alata inhibits the normal development of light-brown apple moth, Epiphyas postvittana in transgenic apple plants. Plant Cell Rep. 26, 773-82.

Johnson, E.D., Miller, E.A. and Anderson, M.A. (2007) Dual location of a family of proteinase inhibitors within the stigmas of Nicotiana alata. Planta 225: 1265-1276.

Charity, J.A., Hughes, P., Anderson, M.A., Bittisnich, D.J., Whitecross, M. and Higgins, T.J.V. (2005) Pest and disease protection conferred by expression of barley ß-hordothionin and Nicotiana alata proteinase inhibitor genes in transgenic tobacco. Functional Plant Biology 32, 35-44.

Korsinczky, M.L.J., Schirra, H.J., Rosengren, K.J., West, J., Condie, B.A., Otvos, L., Anderson, M.A. and Craik, D.J. (2001) Solution structures by 1H NMR of the novel cyclic trypsin inhibitor SFTI-1 from sunflower seeds and an acyclic permutant. J. Mol. Biol. 311: 579-591.

Schirra, H. J., Scanlon, M. J., Lee, M. C. S., Anderson, M. A. and Craik, D. J. (2001) The solution structure of C1-T1, a two-domain proteinase inhibitor derived from a circular precursor protein from Nicotiana alata. Journal of Molecular Biology 306, 69-79.

Miller, E. A., Lee, M. C. S., Atkinson, A. H. O. and Anderson, M. A. (2000) Identification of a novel four-domain member of the proteinase inhibitor II family from the stigmas of Nicotiana alata. Plant Molecular Biology 42, 329-333.

Miller, E. A., Lee, M. C. S. and Anderson, M. A. (1999) Identification and characterization of a prevacuolar compartment in stigmas of Nicotiana alata. Plant Cell 11, 1499-1508.

Lee, M. C. S., Scanlon, M. J., Craik, D. J. and Anderson, M. A. (1999) A novel two-chain proteinase inhibitor generated by circularization of a multidomain precursor protein. Nature Structural Biology 6, 526-530.

Miller, E. A. and Anderson, M. A. (1999) Reply: The role of BP-80 in sorting to the vacuole in stigmas. Plant Cell 11, 2071-2073.

Scanlon, M. J., Lee, M. C. S., Anderson, M. A. and Craik, D. J. (1999) Structure of a putative ancestral protein encoded by a single sequence repeat from a multidomain poteinase inhibitor gene from Nicotiana alata. Structure with Folding and Design 7, 793-802.

Charity, J. A., Anderson, M. A., Bittisnich, D. J., Whitecross, M. and Higgins, T. J. V. (1999) Transgenic tobacco and peas expressing a proteinase inhibitor from Nicotiana alata have increased insect resistance. Molecular Breeding 5, 357-365.

Miller, E. A. and Anderson, M. A. (1999) Uncoating the mechanisms of vacuolar protein transport. Trends in Plant Science 4, 46-48.

Anderson, M.A., Van Heeswijck, R., West, J., Bateman, K, Lee, M., Christeller, J. T., McDonald, G. and Heath, R. L. (1997) Proteinase inhibitors from Nicotiana alata enhance plant resistance to insect pests. Journal of Insect Physiology 43, 833-842.

Nielsen, K. J., Hill, J. M., Anderson, M. A. and Craik, D. J. (1996) Synthesis and structure determination by NMR of a putative vacuolar targeting peptide and model of a proteinase inhibitor from Nicotiana alata. Biochemistry 35, 369-378.

Nielsen, K. J., Heath, R. L., Anderson M. A. and Craik, D. J. (1995) Structures of a series of 6-kDa trypsin inhibitors isolated from the stigma of Nicotiana alata. Biochemistry 34,14304-14311.

Heath, R. L., Barton, P. A., Simpson, R. J., Reid, G. E., Lim, G. and Anderson, M. A. (1995) Characterization of the protease processing sites in a multidomain proteinase inhibitor precursor from Nicotiana alata. European Journal of Biochemistry 230, 250-257.

Nielsen, K. J., Heath, R. L., Anderson, M. A. and Craik, D. J. (1994) The three-dimensional solution structure by ¹H NMR of a 6-kDa proteinase inhibitor isolated from the stigma of Nicotiana alata. Journal of Molecular Biology 242, 231-243.

Atkinson, A. H., Heath, R. L., Simpson, R. J., Clarke, A. E. and Anderson, M. A. (1993) Proteinase inhibitors in Nicotiana alata stigmas are derived from a precursor protein which is processed into five homologous inhibitors. Plant Cell 5, 203-213.

Plant Defensins

van der Weerden, N.L., Lay, F.T. and Anderson, M.A. (2008) The plant defensin, NaD1, enters the cytoplasm of Fusarium oxysporum hyphae. J. Biol. Chem. 283, 14445-14452.

Pelegrini, P.B., Lay, F.T., Murad, A.M., Anderson, M.A. and Franco, O.L. (2008) Novel insights on the mechanism of action of alpha-amylase inhibitors from the plant defensin family. Proteins. 73, 719-729.

Lay, F.T. and Anderson, M.A. (2005) Defensins – Components of the innate immune system in plants. Current Protein and Peptide Science 6, 85-101.

Janssen, B. J., Schirra, H. J., Lay, F. T., Anderson, M. A. and Craik, D. J. (2003) Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds. Biochemistry 42, 8214-8222.

Lay, F. T., Brugliera, F. and Anderson, M.A. (2003) Isolation and properties of floral defensins from ornamental tobacco and petunia. Plant Physiology 131, 1283-1293.

Lay, F. T., Schirra, H. J., Scanlon, M. J., Anderson, M. A., Craik, D. J. (2003) The three-dimensional solution structure of NaD1, a floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP. Journal of Molecular Biology 325, 175-188.

CYCLOTIDES

Craik, D.J., Anderson, M.A., Barry, D.G., Clark, R.J., Daly, N., Jennings, C.V. & Mulvenna, J. (2002) Discovery and structures of cyclotides: novel macrocyclic peptides from plants. Letters in Peptide Science 8: 119-128.

Craik, D., Anderson, M.A. & Daly, N. (2003) Circular proteins tied in knots. Todays Life Sciences 15: 28-32.

Gunasekera, S., Daly, N.L., Anderson, M.A. and Craik, D.J. (2006) Chemical synthesis and biosynthesis of the cyclotide family. IUBMB Life 58, 1-10.

Gruber, C.W., Čemažar, M., Anderson, M.A. and Craik, D.J. (2007) Insecticidal plant cyclotides and related cystine knot toxins. Toxicon 49, 561-575.

Jennings, C., West, J., Waine, C., Craik, D. and Anderson, M.A. (2001) Biosynthesis and insecticidal properties of plant cyclotides-the cyclic knotted proteins from Oldenlandia affinis. Proc. Natl. Acad. Sci. USA 98: 10614-10619.

Dutton, J.L., Renda, R.F., Waine, C., Clark, R.J., Daly, N.L., Jennings, C.V., Anderson, M.A. and Craik, D.J. (2004). Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. J. Biol. Chem. 279, 46858-46867.

Jennings, C.V., Rosengren, J., Daly, N.L., Plan, M., Stevens, J., Scanlon, M.J., Waine, C., Norman, D.G., Anderson, M.A. and Craik, D.J. (2005). Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist; Do Möbius strips exist in nature? Biochemistry 44, 851-860.

Mulvenna, J.P., Bharathi, R., Mylne, J.S., Burton, R.A., Shirley, N.J., Fincher, G.B., Anderson, M.A. and Craik, D.J. (2006) Discovery of cyclotide –like protein sequences in graminaceous crop plants; Ancestral precursors of circular proteins? The Plant Cell, 18, 1-11.

Gruber, C.W., Čemažar, M., Clark, R.J., Horibe, T., Renda, R.F., Anderson, M.A. and Craik, D.J. (2007) A novel plant protein disulphide isomerise involved in the oxidative folding of cystine knot defense proteins. J. Biol. Chem. 282 (28), 20435-46.

Saska, I., Gillon, A.D., Hatsugai, N., Dietzgen, R.G., Hara-Nishimura, I., Anderson, M.A. and Craik, D.J. (2007). An asparaginyl endopeptidase mediates in vivo protein backbone cyclisation.  J. Biol. Chem. 282 (40), 29721-28.

Barbeta, B.L., Marshall, A.T., Gillon, A.D., Craik, D.J. and Anderson, M.A. (2008) Plant cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae. Proc Natl Acad Sci. 105, 1221-1225.

Gillon, A.D., Saska, I., Jennings, C.V., Guarino, R.F., Craik, D.J. and Anderson, M.A. (2008) Biosynthesis of circular proteins in plants. Plant Journal. 53, 505-515.

Saska, I., Colgrave, M.L., Jones, A., Anderson, M.A. and Craik, D.J. (2008) Quantitative analysis of backbone-cyclised peptides in plants. J. Chrom. A. (In press)